Vitamin D, 25-Hydroxy
$ 55.99
Vitamin D, 1,25-Dihydroxy LC/MS/MS Blood Test
Active Vitamin D Calcitriol Test for Calcium, Kidney and Parathyroid Evaluation
The Vitamin D, 1,25-Dihydroxy LC/MS/MS Blood Test measures the active form of vitamin D in the blood, also known as calcitriol.
This test is best used when a healthcare provider specifically requests active vitamin D testing for calcium metabolism, kidney-related vitamin D processing, parathyroid concerns, or other focused medical evaluation.
Important: This is not the standard vitamin D deficiency test. If you want to check routine vitamin D deficiency or supplement status, you likely need the Vitamin D, 25-Hydroxy test instead.
✔ Measures active vitamin D, also called calcitriol
✔ LC/MS/MS testing method
✔ Useful when specifically requested by your provider
✔ Doctor's order included
✔ No insurance required
⏱ Typical turnaround: results are usually emailed in 1–3 days.
What This Test Measures
This blood test measures 1,25-dihydroxyvitamin D, the active hormone form of vitamin D. Vitamin D is first converted in the liver to 25-hydroxyvitamin D and then converted mainly in the kidneys to 1,25-dihydroxyvitamin D.
- Test name: Vitamin D, 1,25-Dihydroxy LC/MS/MS
- Also known as: Calcitriol, 1,25-Dihydroxy Vitamin D, 1,25 di-OH Vitamin D
- Sample type: Blood
- Purpose: Active vitamin D evaluation
Vitamin D 1,25 vs Vitamin D 25-Hydroxy
- Vitamin D, 25-Hydroxy - Best for routine vitamin D deficiency, sufficiency, and supplement monitoring.
- Vitamin D, 1,25-Dihydroxy - Best for active vitamin D evaluation related to calcium, kidney, parathyroid, hypercalcemia, or special provider-directed testing.
A person can have a normal Vitamin D, 1,25-Dihydroxy result even when overall vitamin D stores are low. If you are unsure which vitamin D test you need, confirm with your healthcare provider before ordering.
Why Order This Test?
- Provider specifically requested it - This test is most appropriate when your provider asks for active vitamin D or calcitriol testing.
- Calcium metabolism evaluation - May be used when calcium levels are abnormal.
- Kidney-related evaluation - May be used when kidney function affects vitamin D activation.
- Parathyroid-related evaluation - May help support review of parathyroid-related concerns.
- Specialized vitamin D workup - May be used in focused bone, mineral, or metabolic evaluations.
Who May Consider This Test?
- Individuals whose provider requested Vitamin D, 1,25-Dihydroxy testing
- Patients being evaluated for certain calcium abnormalities
- Patients being evaluated for parathyroid-related concerns
- Individuals with kidney-related vitamin D metabolism concerns
- Patients being monitored for specific bone or mineral metabolism concerns
Important Limitations
- This test does not diagnose vitamin D deficiency by itself.
- This test does not replace Vitamin D, 25-Hydroxy for routine deficiency screening.
- A normal result does not rule out low vitamin D stores.
- This test does not diagnose kidney disease, parathyroid disease, sarcoidosis, lymphoma, or any medical condition by itself.
- Results should be reviewed with your healthcare provider along with calcium, kidney function, parathyroid hormone, symptoms, and other lab findings.
Before Ordering
- Confirm that your provider requested Vitamin D, 1,25-Dihydroxy, not Vitamin D, 25-Hydroxy.
- If your goal is routine vitamin D deficiency screening, consider ordering Vitamin D, 25-Hydroxy instead.
- Do not stop supplements or medications unless your healthcare provider tells you to.
- If you are unsure which vitamin D test is right for you, ask your healthcare provider before ordering.
How It Works
- Order online.
- Receive your lab order. A doctor's order is included.
- Visit a participating lab location.
- Complete a simple blood draw.
- Receive your results. Result timing may vary by lab, location, and processing requirements.
Why Choose This Test?
✅ Measures active vitamin D, also called calcitriol
✅ Useful for provider-directed calcium, kidney, or parathyroid evaluation
✅ Doctor's order included
✅ No insurance needed
✅ Convenient online ordering
✅ Results can be reviewed with your healthcare provider
Frequently Asked Questions
What does this test check?
This test checks Vitamin D, 1,25-Dihydroxy, the active form of vitamin D also called calcitriol.
Is this the regular vitamin D test?
No. The regular vitamin D test used for most deficiency screening is Vitamin D, 25-Hydroxy.
Should I order this for vitamin D deficiency?
Usually no. Vitamin D, 25-Hydroxy is generally the preferred test for vitamin D deficiency screening.
Why would my provider order this test?
Your provider may order this test for calcium, kidney, parathyroid, bone, or mineral metabolism evaluation.
Do I need to fast?
Fasting is usually not required unless your healthcare provider gives you different instructions.
Is a doctor's order included?
Yes. A doctor's order is included with this lab test.
Order Your Vitamin D, 1,25-Dihydroxy LC/MS/MS Test
Use this test when your provider specifically requests active vitamin D testing for calcitriol, calcium metabolism, kidney-related concerns, parathyroid-related concerns, or other focused medical review.
Order your Vitamin D, 1,25-Dihydroxy LC/MS/MS Blood Test today.
